Ly6g + neutrofil härledd mir-223 hämmar nlrp3-inflammasomen i
CD45 staining was used to distinguish between CD11b + /CD45 low resident microglia (gate P9) and CD11b + /CD45 high /Ly6G - /Ly6C high invading macrophages/monocytes (gates P8, P10, and P11), which were mostly absent in naive brain samples. B) GAMs from RCAS-PDGFb tumors were isolated relying on an antibody-independent approach. 2008-04-01 · 1. Circulation.
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We sequenced RNA in mouse CD11b + Ly6G − Ly6C high and CD11b + Ly6G − Ly6C low monocytes sorted from WT whole blood. Sorted cells (200 000/monocyte subset) were collected in 1400 μL QIAzol Lysis Reagent (Qiagen, Germantown, MD) and total RNA (50–100 ng) was isolated manually per manufacturer’s protocol. (D) Representative FACS plots of CD11b+,Ly6G+Ly6C- neutrophils, Ly6C high Ly6G- monocytes, and F4/80+ macrophages in the blood of CVB3-infected mice treated with anti-Ly6G or anti-Gr1 on day7 p.i. (pre The following markers were used: CD45 low , CD11b + , F4/80 + , Ly6C − , Ly6G − for microglia, and CD45 high , CD11b + , F4/80 + , Ly6C − , Ly6G − for macrophages, relative to total viable single cells in dissociated retina. Data are expressed as mean ± SEM (WT, n = 4; Prcd −/− , n = 5; P23H, n = 2). About InVivoMAb anti-mouse Ly6G/Ly6C. The NIMP-R14 monoclonal antibody is specific for murine neutrophils.
Molecular Regulation of Inflammation and - DiVA
Circulation. 2008 Apr 1;117(13):1649-57.
Tumör-associerade makrofagers inverkan vid - CORE
Siglec-F+/–. Ly-6G+/–. CD11c+/–.
Dotted line: Isotype control antibody.
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Here, we focused on Ly6C- macrophages, which are derived from the embryonic yolk sac and post-development become resident in the kidneys. We found that C-C chemokine receptor type 2 (CCR2) deficiency, which blocks the migration of Ly6C+ macrophages from the bone marrow to the sites of injury, alleviated ischemia-induced AKI in mice. Neutrophils were defined as CD11b + Ly6C lo CX 3 CR1-GFP − Ly6G + cells, while monocytes were defined as CD11b + Ly6C hi CX 3 CR1-GFP + MHCII − Ly6G − cells. KC and MoMF expressed similar levels of the macrophage markers F4/80, CD64 (FcγR), and MHCII, but could clearly be discriminated according to presence and absence of the CX 3 CR1 Ly6C, and Ly6G have long been used to identify various splenic cell myeloid popula-tions.
1A and D). Remark-ably, distinct subsets of TAMs were clearly distinguishable: Ly6C intMHC IIhi (Ly6C TAMs, gate 2), Ly6ClowMHC IIhi (MHC IIhi TAMs, gate 3), and Ly6ClowMHC IIlow (MHC IIlow TAMs,gate4).ThemajorityofLy6ClowMHC II− cells were CCR3
CD11b+Ly6C high (Ly6Chi) cells (inflammatory monocytes) and CD11b+Ly6C low cells (reparative monocytes) were recruited into the liver following I/R injury. Treatment with clodronate‑loaded liposomes, which transiently deplete systemic macrophages, alleviated hepatic damage.
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DEX increases the F4/80 + Ly6G + macrophage population in vivo. Panel A and B. In vivo the F4/80 + Ly6G + macrophage, F4/80 + Ly6G-macrophage, and F4/80-Ly6G + neutrophils shown by FACS detection; Panel C. quantitation data.